Circular Template Matching for Cell Segmentation

Hi All,
I am working on Cell Segmentation for the detection of cancer cells. The cells are separated from the background using a threshold. Later form factor is used to separate the circular cells from the rest of the non-circular regions in the image. However, some of the cells go undetected as there are a couple of pixels missing to form the complete circular shape. So I wanted to use a circular template to detect the cell regions. Could some one help me with the MATLAB code for this. It is a little urgent,so any help is appreciated! Thanks in advance.

Regards,
Kumar Start a New Thread

A hough transform might do the trick, although I understand it requires a fair amount of processing. The shape you're looking for needs to be expressed parametrically then a lot of different combinations of the parameters are tested against your thresholded image. A maximum will occur where your object is even with breaks in the circle.

Hope that helps,

Mike. Start a New Thread

you may also try watershed segmentation. you can find more information
in matlab help file. Start a New Thread