Hi all,
I am a newbie in MATLAB. I am trying to do particle analysis on an image in
MATLAB.I work with nanocrystals of varying sizes and contrast. Basically what I
am trying to do is count the number of cells in the image by setting
thresholds and make measurements like area, perimeter, radius. The challenge
with this work is that the nano crystals change both in contrast and circularity
from image to image.
Has anybody worked on this before? Does anybody know what algorithm works best
in this case.
Please let me know. It will really help me.
Thanks,
Sandhya
Particle analysis
Started by ●May 27, 2008
Reply by ●May 28, 20082008-05-28
I guess you must be using regionprops to calculate the area, perimeter etc.
You can know the count of number of cells crossing the threshold by using
the
statement [L,num]=bwlabel(matrix) before you do regionprops which you need
to do anyways to convert the matrix to a binary value. the variable num
gives the number of cells identified or ones which crossed the threshold.
This is to be done after you apply the condition for threshold.
On 27 May 2008 22:00:49 -0000, sandhya nageswaren
wrote:
> Hi all,
> I am a newbie in MATLAB. I am trying to do particle analysis on an image in
> MATLAB.I work with nanocrystals of varying sizes and contrast. Basically
> what I am trying to do is count the number of cells in the image by setting
> thresholds and make measurements like area, perimeter, radius. The challenge
> with this work is that the nano crystals change both in contrast and
> circularity from image to image.
> Has anybody worked on this before? Does anybody know what algorithm works
> best in this case.
> Please let me know. It will really help me.
>
> Thanks,
> Sandhya
You can know the count of number of cells crossing the threshold by using
the
statement [L,num]=bwlabel(matrix) before you do regionprops which you need
to do anyways to convert the matrix to a binary value. the variable num
gives the number of cells identified or ones which crossed the threshold.
This is to be done after you apply the condition for threshold.
On 27 May 2008 22:00:49 -0000, sandhya nageswaren
wrote:
> Hi all,
> I am a newbie in MATLAB. I am trying to do particle analysis on an image in
> MATLAB.I work with nanocrystals of varying sizes and contrast. Basically
> what I am trying to do is count the number of cells in the image by setting
> thresholds and make measurements like area, perimeter, radius. The challenge
> with this work is that the nano crystals change both in contrast and
> circularity from image to image.
> Has anybody worked on this before? Does anybody know what algorithm works
> best in this case.
> Please let me know. It will really help me.
>
> Thanks,
> Sandhya